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產品名稱：β-Glucosyltransferaseenzyme
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產品廠商：ActiveMotif
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β-Glucosyltransferaseenzyme

詳情介紹：

Product details
Product details
Characteristics	β-Glucosyltransferase will transfer the glucose moiety from the UDP-Glucose (uridine diphosphoglucose) to 5-hydroxymethylcytosine residues in double-stranded DNA.
Components	200,000 Units β-Glucosyltransferase enzyme 10X β-Glucosyltransferase Reaction Buffer 50 mM UDP-Glucose 1 M DTT

Target details
Target details

Application Details
Application Details
Application Notes	The β-Glucosyltransferase enzyme can be used to glucosylate 5-hmC DNA. Glucosylated DNA can be used as a differentiator with glucosyl-sensitive restriction enzymes. The use of a labeled [14C] UDP-Glucose donor would enable labeling of the 5-hmC residues.
Comment	One unit is defined as the amount of enzyme required to fully protect 0.5 μg of 5-hmC DNA Standard (Catalog No. 55008) in 1 hour at 37°C in a total reaction volume of 50 μl from cleavage by 4 units of GlaI restriction enzyme in an incubation of 16 hours at 30°C. A pilot titration of enzyme is recommended for protection of genomic DNA.
Restrictions	For Research Use only

Handling
Handling
Storage	-20 °C/-80 °C
Storage Comment	Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Images
Images
Supplier Images	Figure 1: 0.5 μg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated... Figure 1: 0.5 μg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated in the absence or presence of 1 unit β-Glucosyltransferase enzyme for 1 hour at 37°C. Following column purification, reactions were treated with 4 units of glucosyl-sensitive restriction enzyme GlaI and run on an 2.5% agarose gel. Figure 1: 0.5 μg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated in the absence or presence of 1 unit β-Glucosyltransferase enzyme for 1 hour at 37°C. Following column purification, reactions were treated with 4 units of glucosyl-sensitive restriction enzyme GlaI and run on an 2.5% agarose gel.

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