The antibody-conjugated bead set is designed to be used in conjunction with 's Histone H3 PTM Multiplex Kit . Follow the recommendations in the Histone H3 PTM Multiplex Kit manual for the preparation and use of the antibody-conjugated bead set. Each analyte specific antibody-conjugated bead contains its own unique fluorescent label allowing the user to distinguish different bead sets within a single sample for multiplex experiments. The beads can be read and identified using a Luminex 200?, MAGPIX? or FLEXMAP 3D? instrument and xPONENT? software. The associated bead region for the Histone H3K27me3 Ab-conjugated bead set is #57. The recommended detection limit for this bead set is: Acid extracts - use greater than 32 ng per well of sample Purified histones - use greater than 4 ng per well of sample