99国产精品视频免费观看_国产一区毛片_久久激情视频免费观看_国产成人aa在线观看网站站

產品詳情
所在位置: 首頁> 產品目錄> 輔助試劑>
  • 產品名稱:Caspase-2,3Substrate-pNA

  • 產品型號:
  • 產品廠商:KamiyaBiomedical
  • 產品文檔:
你添加了1件商品 查看購物車
簡單介紹:
Caspase-2,3Substrate-pNA
詳情介紹:
Purpose Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in DMSO, and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 X 10^6 /mL.
2. Assay 0.01 mL cell lysate in a final volume of 0.1 mL. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37°C for 0-3 hr. Take periodic readings of absorbance at 405 nm.
Sequence Acetyl-Val-Asp-Val-Ala-Asp-pNA, Ac-VDVAD-pNA
Specificity Substrate for caspase-2, 3.
Purity > 97 % by HPLC
Background Chromogenic paranitroanilide-peptide substrate for caspase-2 (ICH-1). Release of free pNA is monitored by absorbance at 405 nm (epsilon=9,160 M^-1 cm^-1 ). Caspase-2 (also known as ICH-1) is a member of the caspase family of cysteine proteases involved in apoptosis. It is a member of the Group II caspases, along with caspases-3 and - 7. Group II caspases prefer peptides of the DEXD-type as substrates. However, unlike caspases-3 and -7, Caspase-2 requires a P5 amino acid in the peptide for efficient cleavage. The similar substrate specificities of the Group II caspases suggests that their roles in cells are at least overlapping, if not completely redundant. The requirement for a fifth amino acid in substrates for Caspase-2 means that the DEVD-type, while serving as substrates for caspases-3 and -7, do not work with Caspase-2. For this reason, the Ac-VDVAD-pNA substrate is excellent for studying Caspase-2.
Molecular Weight 679 Da
Application Notes Assay of caspase activity in cell extracts.
Restrictions For Research Use only
Format Lyophilized
Storage RT
Background publications Talanian, Quinlan, Trautz, Hackett, Mankovich, Banach, Ghayur, Brady, Wong: "Substrate specificities of caspase family proteases." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 9677-82, 1997 (PubMed).

Thornberry, Rano, Peterson, Rasper, Timkey, Garcia-Calvo, Houtzager, Nordstrom, Roy, Vaillancourt, Chapman, Nicholson: "A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 29, pp. 17907-11, 1997 (PubMed).

Duan, Orth, Chinnaiyan, Poirier, Froelich, He, Dixit: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli, Robertson, Armstrong, Wang, Trapani, Tomaselli, Litwack, Alnemri: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Faucheu, Diu, Chan, Blanchet, Miossec, Hervé, Collard-Dutilleul, Gu, Aldape, Lippke: "A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells." in: The EMBO journal, Vol. 14, Issue 9, pp. 1914-22, 1995 (PubMed).

Kamens, Paskind, Hugunin, Talanian, Allen, Banach, Bump, Hackett, Johnston, Li: "Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15250-6, 1995 (PubMed).

Munday, Vaillancourt, Ali, Casano, Miller, Molineaux, Yamin, Yu, Nicholson: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).

主站蜘蛛池模板: 肥乡县| 富锦市| 土默特右旗| 荣成市| 怀远县| 岳池县| 泽普县| 唐河县| 长汀县| 安康市| 鄂托克前旗| 鄂尔多斯市| 通城县| 西乡县| 额济纳旗| 宝兴县| 洪江市| 赞皇县| 莱西市| 方城县| 乌海市| 眉山市| 平利县| 深圳市| 和龙市| 什邡市| 大兴区| 孟村| 商城县| 鄂托克前旗| 牙克石市| 堆龙德庆县| 西丰县| 广汉市| 上思县| 宝应县| 康定县| 杭锦后旗| 南澳县| 平乡县| 仲巴县|