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  • 產品名稱：Caspase-2,3FluorogenicSubstrate

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  • 產品廠商：KamiyaBiomedical
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簡單介紹：
Caspase-2,3FluorogenicSubstrate
詳情介紹：
Purpose Fluorometer calibration: The fluorometer is calibrated using known concentrations of free AFC (Excitation = 400 nm, Emission = 505 nm) to generate a standard curve of fluorescence versus μ-moles AFC. Samples: Can be either purified or partially purified enzyme preparations. Application to crude cell lysates has not been confirmed. If crude cell lysates are to be assayed, the non- specific protease background must be determined using our Caspase-2,3 Inhibitor.
Sequence Ac-Val-Asp-Val-Ala-Asp-AFC, Ac-VDVAD-AFC
Specificity Highly specific substrate for Caspase-2. Will also serve as a substrate for Caspases-3 and -7.
Background Peptide substrate labeled at the carboxy end with AFC (7-amino-4-trifluoromethyl coumarin). Designed to measure Caspase-2 activity in vitro. Caspase-2 (also known as ICH-1) is a member of the caspase family of cysteine proteases involved in apoptosis. It is a member of the Group II caspases, along with caspases-3 and - 7. Group II caspases prefer peptides of the DEXD-type as substrates. However, unlike caspases-3 and -7, Caspase-2 requires a P5 amino acid in the peptide for efficient cleavage. The similar substrate specificities of the Group II caspases suggests that their roles in cells are at least overlapping, if not completely redundant. The requirement for a fifth amino acid in substrates for Caspase-2 means that the DEVD-type, while serving as substrates for caspases-3 and -7, do not work with Caspase-2. For this reason, the Ac-VDVAD-AFC substrate is excellent for studying Caspase-2.
Molecular Weight 770 Da
Application Notes For in vitro assays of Caspase-2 activity, or Caspases-3 and -7 although the Caspase-3 Fluorogenic Substrate (Ac-DEVD-AFC, Cat. No. AC-003) is preferable for these two enzymes. The Caspase-2,3 Fluorogenic Substrate can be used with purified or partially purified enzymes or possibly with crude cell lysates (if the Caspase-2,3 Inhibitor is included to determine background protease activity).
Restrictions For Research Use only
Storage RT
Background publications Talanian, Quinlan, Trautz, Hackett, Mankovich, Banach, Ghayur, Brady, Wong: "Substrate specificities of caspase family proteases." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 9677-82, 1997 (PubMed).

Thornberry, Rano, Peterson, Rasper, Timkey, Garcia-Calvo, Houtzager, Nordstrom, Roy, Vaillancourt, Chapman, Nicholson: "A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 29, pp. 17907-11, 1997 (PubMed).

Wang, Miura, Bergeron, Zhu, Yuan: "Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death." in: Cell, Vol. 78, Issue 5, pp. 739-50, 1994 (PubMed).

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