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  • 產品名稱：CollaGelHydrogelSoft

  • 產品型號：
  • 產品廠商：OlafPharmaceuticals
  • 產品文檔：
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CollaGelHydrogelSoft
詳情介紹：
Characteristics CollaGel Hydrogel Soft is a biocompatible complex of Type I Collagen fibers that will help accelerate the pace of your biomedical and cell/3Dtissue engineering applications. CollaGel Hydrogel Soft contains our high quality, sterile Type I Rat Tendons Collagen which has been specially formulated for ease of gel formation. Once in a 3D tissue model, the CollaGel Hydrogel Soft will not break or tear apart easily when stretched. CollaGel Hydrogel Soft can also be flow able, allowing it to be readily used as an injectable, biocompatible drug delivery matrix on animal models. To control release of your drug, it is necessary to bind your active agent to CollaGel Hydrogel Soft, either by covalent or non-covalent bonds, or by sequestering in a secondary matrix. Other potential applications of CollaGel Hydrogel Soft are as orthopedic adhesives via their swelling ability, as scaffolds for bone infiltration and formation through their mesh structure, as isolators to retain cells, or as gene delivery complexes.
Source: Rat Tendons
Conductivity:1.5 mg/ml (Sircol Assay)
Purity > 95 % SDS PAGE
Endotoxin Level < =1EU/ml
Components 3x106 cells or a fully confluent T75
20 ml CollaGel Hydrogel Soft
DMEM
Protocol Thaw your CollaGel Hydrogel Soft sample bottle at room temp or in a 37°C water bath. When you see a small amount of ice left in your sample, transfer the bottle into an ice bath. It is important to keep CollaGel Hydrogel on ice since it will solidify at temperatures above 8°C.
Place a sterile magnetic stir bar in a sterile beaker.
Place plate containing ice on a stir plate.
Place the sterile beaker containing the stir bar on the ice bath.
Pour you CollaGel Hydrogel Softinto the beaker carefully, try to avoid air bubbles.
Slowly start stirring CollaGel Hydrogel Soft solution.
Add your wanted media to the CollaGel Hydrogel Soft solution. Judge the pH visually by the phenol red in the media, add your cell suspension.
Pipette your wanted volume of the mixture into each well.
Let 6-well plate sit at room temp for 10-15 minute before placing in the incubator.
One Hour later add your wanted volume of media on top of each CollaGel Hydrogel Soft (2-2.5ml).
Use within 1 week, change media every 2 days or as needed.
Restrictions For Research Use only
Format Liquid
Concentration 1.5 mg/mL
Storage -20 °C
Expiry Date 12 months
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