The antibody-conjugated bead set is designed to be used in conjunction with 's Histone H3 PTM Multiplex Kit . Follow the recommendations in the Histone H3 PTM Multiplex Kit manual for the preparation and use of the antibody-conjugated bead set. Each analyte specific antibody-conjugated bead contains its own unique fluorescent label allowing the user to distinguish different bead sets within a single sample for multiplex experiments. The beads can be read and identified using a Luminex 200?, MAGPIX? or FLEXMAP 3D? instrument and xPONENT? software. The associated bead region for the Histone H3K27ac Ab-conjugated bead set is #26. The recommended detection limit for this bead set is: Acid extracts - use greater than 250 ng per well of sample Purified histones - use greater than 7.8 ng per well of sample Recommendations for Dilution of the Biotinylated Histone H3 Antibody: H3K27ac Ab-conjugated beads may benefit from the use of Biotinylated Histone H3 antibody at higher concentrations in order to increase the reporter signal. If the H3K27ac Ab-conjugated bead generates MFI values less than 1000 in 1 μg lysate, it is recommended to use the Biotinylated Histone H3 reporter antibody at 1:250 dilution. Please refer to the Buffer Preparation and Recommendations section in the manual for the Histone H3 PTM Multiplex Kit for detailed instructions on diluting the Biotinylated Histone H3 antibody for use in the assay.