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  • 產品名稱：HistoneH3TotalAb-conjugatedBeads

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HistoneH3TotalAb-conjugatedBeads
詳情介紹：
Specificity The Histone H3 Total Ab-conjugated Beads contain Luminex? magnetic microspheres that have been conjugated with antibodies directed against total Histone H3.
Characteristics Histone H3 PTM Multiplex Assay is a novel high throughput bead-based ELISA assay designed for use with the MAGPIX?, Luminex? 200? or FLEXMAP 3D? instruments to enable simultaneous interrogation of the levels of multiple histone H3 PTMs within the same sample. The 3-hour assay is ideally suited for high-throughput screening and profiling of histone modification levels of clinical or compound-treated samples. The multiplexing ability means you can simultaneously screen for on-target and off-target effects within the same sample. This kit offers several advantages over traditional methods (e.g. Western Blotting) including faster turnaround times and the ability to utilize smaller sample amounts.
Alternative Name Histone h3
Application Notes Optimal working dilution should be determined by the investigator.
Comment

The antibody-conjugated bead set is designed to be used in conjunction with 's Histone H3 PTM Multiplex Kit . Follow the recommendations in the Histone H3 PTM Multiplex Kit manual for the preparation and use of the antibody-conjugated bead set. Each analyte specific antibody-conjugated bead contains its own unique fluorescent label allowing the user to distinguish different bead sets within a single sample for multiplex experiments. The beads can be read and identified using a Luminex 200?, MAGPIX? or FLEXMAP 3D? instrument and xPONENT? software. The associated bead region for the Histone H3 Total Ab-conjugated bead set is #66. The recommended detection limit for this bead set is: Acid extracts - use greater than 30 ng per well of sample Purified histones - use greater than 1 ng per well of sample
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment Beads should be stored at +4°C. Do not freeze. The Positive Control should be stored at -20°C.
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