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Comment
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The cocktail solution inhibits aminopeptidases and cysteine, serine and aspartic acid proteases. Protease Inhbitor Cocktail is provided EDTA-free, as a ready-to-use 100X stock solution in DMSO. The cocktail composition and inhibitor targets are listed below:
- AEBSF, 104 mM - serine proteases
- Aprotinin, 80 μM - serine protease
- Bestatin, 4 mM - aminopeptidases
- E-64, 1.4 mM - cysteine proteases
- Leupeptin, 2 mM - serine and cysteine proteases
- Pepstatin A, 1.5 mM - aspartic acid proteases
It is recommended to use 10 μl of the cocktail solution for every 1 ml of cell lysate. Since protease levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors.
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