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Purpose
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Ni-NTA Resin is used for one-step purification of Histagged proteins. The His-tagged proteins bind to Ni2+ cations, which are immobilized on the Ni-NTA resin by 4 metal-chelating sites. After unbound proteins are washed away, the target proteins are recovered by gradient elution. It is suitable for both native and denatured protein purification.?
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