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Application Notes
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MEMBRANE DIRECTIONS:
1. Store the reagents, HRP-A and HRP-B, at 4-8°C.
2. Mix equal volumes of HRP-A and HRP-B in a clean container and equilibrate at room temperature for 30 minutes before use.
3. HRP enzyme substrate has a wide range to detect HRP enzyme on a membrane. Lower enzyme detection is at the femto gram level and the highest concentration of HRP enzyme detection may be one nano gram depending on the source of the HRP enzyme.
4. Do not contaminate the HRP enzyme substrate with HRP enzyme or other proteins.
5. For good results, wash your tube, microtiter plate or membrane with 0.2M phosphate buffer, pH 8.4 to 8.6, and then use the substrate.
6. Best results for chemiluminescence can be obtained from 5 minutes to 30 minutes after contacting substrate with HRP enzyme.
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