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Purpose
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Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in high purity (>99.9%) DMSO, and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 x 10^6 /mL.
2. Assay 0.01 mL cell lysate in a final volume of 0.1 mL. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37°C for 0-3 hr. Take periodic readings of absorbance at 405 nm.
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