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Purpose
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Superparamagnetic silica particles with modified surface for SH coupling chemistry.
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Specificity
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Coating chemistry: Sulfydryl
Bead concentration: 8 - 20 x 10^9 beads/mL
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Characteristics
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MagSi-Tools are surface activated magnetic particles, intended for covalent immobilization of proteins (e.g. antibodies, enzymes), peptides, nucleic acids or other molecules of interest. Different surface modifications and bead sizes allow for choosing the optimal product for the right molecule to be coupled, and for the intended application. Please take into consideration which groups are available on the ligand for coupling, and try to prevent inactivation or hiding the active or exposed site of the ligand.
After coupling the molecule of interest (ligand) is coupled to the magnetic particles, the resulting beads can be used in downstream applications such as:
- Isolating specific target proteins, antibodies, nucleic acids, cells, viruses, etc. (preparative applications)
- Detecting specific target proteins, nucleic acids, cells, viruses, etc. (diagnostic applications)
- Immobilizing enzymes, thereby enhancing stability and minimizing auto-catalysis. Magnetic collection of the particle/enzyme complex allows to remove the enzyme from the reaction, and to reuse it in a new reaction.
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Components
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Magnetic silica beads with activated surface
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Material not included
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Buffers and Materials (depending on the application)
Magnetic separator for bead separation/collecting
Mixer/vortex to homogenize samples and resuspend beads (depending on the application)
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ProductDetails: Bead Ligand
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Surface for SH Coupling Chemistry
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ProductDetails: Bead Matrix
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Superparamagnetic Silica particles
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ProductDetails: Bead Size
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Bead size: 600 nm
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