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產品名稱：ECLsubstrate(Mark-ItGlowliquid)
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產品廠商：Micromol
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ECLsubstrate(Mark-ItGlowliquid)

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Product details
Product details
Characteristics	The Mark-It Glow product line represents a series of novel very sensitive two component substrates for the development of Western-Blots based on the enhanced luminol technique catalysed by horseradish peroxidase (HRP) conjugated antibodies/secondary reagents. Mark-It Glow is available as standard formulation Mark-It Glow Liquid, which is suitable for the development of any standard Western Blot with a sensitivity in the picomolar range. In addition, with the Mark-It Glow Ultra formulation we offer a second system with an extremely sensitive detection limit enabling the detection of even low abundant cellular proteins in the femtomolar region. Unlike other substrates, you don't have to mix Mark-It Glow. You simply position your blot on a plastic sheet and spray as much as you need from bottle 1 and then from bottle 2. Besides the ease of use the performance of the reagent is excellent. For users with very high consumption we also provide a maxi-pack of Mark-It Glow Liquid without sprayhead.
Components	100 mL Solution A + 100 mL Solution B in spray flasks (white PE)

Application Details
Application Details
Application Notes	200 mL are sufficient for 10.000 cm2 (ca. 100 mini-gels)
Restrictions	For Research Use only

Handling
Handling
Format	Liquid
Storage	4 °C

Images
Images
Supplier Images	Western Blot developed with Mark-It Glow Liquid ECL substrate. Decreasing amounts of ... ECL substrate Mark-It Glow Liquid is superior to its competitors on the market. Mark-... Images for product: ECL substrate (Mark-It Glow liquid) Western Blot developed with Mark-It Glow Liquid ECL substrate. Decreasing amounts of a control protein were subjected to Western Blotting (Lanes 1-7: 300ng, 100 ng, 30 ng, 10 ng, 3 ng 1 ng, 300 pg). The blocked membrane was probed with a specific primary antibody and bound antibodies were detected with a specific HRP conjugate. Visualization was performed using MicroMol’s Mark-It Glow Liquid ECL substrate. ECL substrate Mark-It Glow Liquid is superior to its competitors on the market. Mark-It Glow Liquid as well as one of the leading ECL substrates on the market were activated by horseradish peroxidase (0.05 nU/ml) and light emission was measured luminometric at the indicated points of time.

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