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產品名稱：Donkeyanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)
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產品廠商：半導體
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簡單介紹：

Donkeyanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)

詳情介紹：

Product details
Product details
Immunogen	Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Isotype	IgG
Specificity	IgG (H&L)
Characteristics	Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Purification	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.

Target details
Target details
Background	Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	1.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Gentamicin sulfate
Handling Advice	Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Images
Images
Supplier Images	Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit... Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none. Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.

References
References
Background publications	Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).

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