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產品名稱：Rabbitanti-GoatIgG(Heavy&LightChain)Antibody(HRP)
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產品廠商：半導體
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簡單介紹：

Rabbitanti-GoatIgG(Heavy&LightChain)Antibody(HRP)

詳情介紹：

Product details
Product details
Immunogen	Anti-Goat IgG (h&L) was produced by repeated immunization with goat IgG whole molecule in rabbit.
Isotype	IgG
Specificity	IgG (H&L)
Characteristics	Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm

Target details
Target details
Background	Synonyms: Goat IgG (H&L) Antibody, Rb-a-Goat IgG (H&L) Peroxidase conjugated, Goat IgG (H&L) Peroxidase Antibody in rabbit, Rabbit IgG(H&L) peroxidase conjugated Secondary Antibody.
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Antibody Anti-Goat IgG (H&L) peroxidase conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	1.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Gentamicin sulfate
Handling Advice	Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Images
Images
Supplier Images	Western Blot of Peroxidase conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG... Western Blot of Peroxidase conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:5,000 for 60 min at RT. Block: 5% BSA-TBS overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none. Western Blot of Peroxidase conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:5,000 for 60 min at RT. Block: 5% BSA-TBS overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none.

References
References
Background publications	Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).

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